Genetic Engineering

History, Importance and fundamentals, Vectors (Plasmid, Cosmid, Bacteriophage, Yeast), DNA extraction and purification, Enzymes in gene cloning, Preparation of DNA enzymatic map, Methods in construct of recombinant DNA, Genomic libraries, cDNA libraries, Selection of specific clone, DNA sequencing, Induction of mutation in genes, Application of recombinant DNA technology in agriculture