Gene Transfer Technology in Plants

Gene and promoters structure in plants, Gene expression regulatory elements, Mechanism of transgene integration, Transient expression and stable transformation of transgene in plants, Reconstructing a chimeric gene, Reporter genes, Selectable marker genes, Gene transfer methods (Biological, Physical and Chemical), Comparison of gene transfer methods, Agrobacterium mediated gene transfer, Microprojectile bombardment, Electroporation, Microinjection and other methods of gene transfer, Analysis of transgenic plants using molecular and histochemical techniques.