Advanced Molecular Genetics

An Introduction to Molecular Genetics, Evolution of Gene Concepts, Genome Complexity, Repetitive Sequences, Calculation of Number and Size of Repetitive Sequences, Relationship between Genome Size and Genetic Complexity, Gene Identification Methods including Transposon Tagging, T-DNA Tagging, Map Based Cloning and Chemical Genetics, TILLING, EcoTILLING and its applications, Methods of preparing physical map  including Restriction Mapping, Optical Mapping, Gel Stretching, Molecular Combing, FISH and STS Mapping, Eukaryotic nuclear genomes including Physical characteristics, Genetic characteristics and Organization, Comparing the structure of prokaryotic and eukaryotic genes, Transposons, Non-nuclear genomes and exchanging genetic materials between organs and nuclous, Regulation of gene expression in eukaryotes, Types of Alternative Splicing models and its role in regulating gene expression, DNA Methylation and its role in regulating the expression of eukaryotic genes, Methods of methylome identification in genome including CRED-RA, MS-AP-PCR, MSRF, MSAP, AIMS and RLGST, The role of Micro-RNA, RNAi and SiRNA in Regulation of gene expression, Flowering genetics and genetic models, Genetics and vernalization including Role of day length, light quality, cold, etc. in determining flowering time.